Participants learn to:
– Derive NSC from hiPSC
– Culture NSC as adherent monolayers and neurospheres
– Characterize and differentiate NSC
– Culture, characterize and differentiate MSC
– Perform cloning assays
– Reprogram hiPSC using Episomal plasmids and Sendi Virus
– Select, prepare and aliquot hiPSC/hESC culture reagents
– Morphologically assess hiPSC/hESC colonies
– Culture hiPSC/hESC under feeder free conditions in mTeSR and NutriStem
– Pick and manually passage hiPSC/hESC colonies
– Passage hiPSC/hESC using Accutase and ReLeSR
– Cryopreserve and thaw hiPSC/hESC
– Prepare embryoid bodies (scraped and spin-EB’s)
– Differentiate hiPSC/hESC to specific germ layers
– Electroporate hiPSC/hESC
– Establish an hiPSC/hESC laboratory
– Perform standard characterization for immunological markers, vector integration and pluripotency
– Quality control monitor hiPSC/hESC during prolonged culture
– Electroporate hiPSC/hESC
– Troubleshoot common problems
– Comply with ESCRO regulations
– Reduce costs through optimized purchasing and laboratory organization

Our streamlined hiPSC protocols have been validated for use with human embryonic stem cells (hESC). The course curriculum is updated annually to reflect current techniques.