Course Listing


Human Pluripotent Stem Cell Techniques

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Level – Professional Working Scientists

Course Information
 Professional Level Industry scientists, postdocs, technicians and research associates
Format/ Shedule Vacation / Summer and Fall
Location Monrovia, CA
Dates April 22nd-26th
Duration 5 Days
Times 10.00am-5.00pm, Monday-Friday
Class Size 12
 Admission Participants are accepted on a first-come-first-served rolling basis up to 1 week before the course start date. Additional questions? Click here to learn more about our general admissions policy or call us at 310-483-3651.
Course Details

Are you thinking of working with human embryonic stem cells or human induced pluripotent stem cells (hPSCs)? Already culturing hPSCs but experiencing problems? Interested in expanding your skill set to include widely sought after stem cell handling techniques? Pathways to Stem Cell Science's course in Human Pluripotent Stem Cell Techniques is the program for you.

Originally developed in 2009 at the USC Stem Cell Core, our well-established workshop has helped hundreds of scientists world wide to work effectively with hPSCs. During this five-day course, participating scientists gain comprehensive training in validated techniques for culturing, freezing and manipulating hPSCs. Optimized for use with both human embryonic stem cells and human induced pluirpotent stem cells, our streamlined protocols will help you to improve work efficiency, save time and reduce costs. We also provide guidance with laboratory set up and regulatory compliance in addition to ongoing support troubleshooting problems after you return to your own laboratory.

Whether you're an experienced stem cell biologist or a trainee scientist our hPSC workshop provides the perfect opportunity for professional development, networking and learning new tricks! Apply today and gain high-quality comprehensive training in easy-to-follow methods taught by academic and industry experts.


Human Pluripotent Stem Cell Techniques provides industry-level hands-on training in the following procedures:

-hPSC Medium preparation
-Feeder dependent culture
-MEF Feeder isolation and preparation
-Feeder free culture (mTeSR, E8 and NutriStem)
-Enzymatic passaging (accutase, collagenase, dispase and ReLeSR)
-Manual passaging and colony picking
-Identifying hPSC colonies
-Cryopreservation and thawing hPSCs
-Preparation of Embryoid Bodies (scraped and spin-EBs)
-Differentiating hPSCs
-Electroporating hPSCs
-hPSC Characterization
-Setting up an hPSC lab
-Troubleshooting problems
-Regulatory compliance
-Quality control monitoring hPSCs

Curriculum is regularly updated to reflect current techniques


-Experienced course instructors (Industry and academic)
-Essential lab techniques (stem cell culture and manipulation)
-Validated protocols and SOPs
-Develop and hone critical lab skills
-Continuing support with troubleshooting problems
-Resume builder – appeal to internships and employers
-Easy registration
-Affordable tuition (50% cheaper than equivalent training providers)
-Lunch and refreshments
-Extensive hands on experience
-Certificate in professional hPSC culture techniques
-Network with other science professionals

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