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Human Induced Pluripotent Stem Cell Techniques

(2 customer reviews)
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Level – Professional Working Scientists


Course Information
 Professional Level Industry scientists, postdocs, technicians and research associates
Format/ Schedule Spring / Winter
Location Torrance, CA
  2023 Dates Spring: May 15th – May 18th; Winter: November 13th – November 16th;
Duration 4 days
Times 10.00am-5.00pm, Monday-Thursday
Class Size 8
 Admission Participants are accepted on a first-come-first-served rolling basis up to 1 week before the course start date. Additional questions? Click here to learn more about our general admissions policy or call us at 310-483-3651.
Course Details

“hiPSC Techniques” provides comprehensive training in validated methods for deriving, culturing, characterizing, and differentiating human induced pluripotent stem cells (hiPSC).


Participants learn to:
– Reprogram hiPSC using Episomal plasmids and Sendi virus
– Select, prepare and aliquot hiPSC/hESC culture reagents
– Morphologically assess hiPSC/hESC colonies
– Culture hiPSC/hESC feeder free in mTeSR, NutriStem and StemFlex
– Pick and manually passage hiPSC/hESC colonies
– Passage hiPSC/hESC using Accutase, ReLeSR and Collagenase
– Cryopreserve and thaw hiPSC/hESC
– Prepare embryoid bodies (scraped and spin-EB’s)
– Differentiate hiPSC/hESC to specific germ layers
– Perform standard characterization for immunological markers, vector integration and pluripotency
– Quality control monitor hiPSC/hESC during prolonged culture
– Electroporate hiPSC/hESC
– Establish an hiPSC/hESC laboratory
– Troubleshoot common problems
– Comply with ESCRO regulations
– Reduce costs through optimized purchasing and laboratory organization

Our streamlined culture protocols have been validated for use with human induced pluripotent stem cells (hiPSC) and human embryonic stem cells (hESC). The course curriculum is updated annually to reflect current techniques.

Feeder-dependent culture methods can be taught upon request for an additional cost. Our scientists can also advise on approaches for deriving hiPSC from non-human species.

Features and Benefits

The program features:
– Experienced instructors with more than 20 years of cell culture experience

– An optimized and regularly updated curriculum that was originally developed in 2009 at the USC stem cell core facility

– Relevant learning experiences for scientists of all skill levels

– Streamlined procedures that will save you time and money

– Validated protocols and SOPs

– Continuing support with troubleshooting problems

– Networking with other science professionals

– Easy registration

– Affordable tuition

– Certificate of completion

We’ve helped hundreds of science professionals from around the world to gain new skills, enhance their resumes and advance in diverse careers!

Reviews (2)

2 reviews for Human Induced Pluripotent Stem Cell Techniques

  1. Wesley Tierney

    This course is interesting, fun, and good training for working with not only human stem cells, but also teaches you good techniques for working with all types of cell culture. Dr. Fox gives students both guidance and freedom to attempt complicated assays within a good teaching environment – Wesley Tierney, 2017 participant.

  2. Dr. Avinash Srivastava, City of Hope

    This was the most informative source of educational and hands-on training I have encountered in Human Pluripotent Stem Cell Techniques. The course coordinator, Dr. Victoria Fox is very knowledgeable, and her solid scientific research background in stem cells offered a good perspective of laboratory and clinical studies. The execution of the program was extremely well organized. It was small enough that the hands-on breakout sessions in the afternoon were intimate enough to fully participate and ask questions freely. This concise 5-day course changed my approach to stem cell acquisition and helped tremendously in my research. I recommend this course to anyone new or experienced in the changing world of stem cell research – Dr. Avinash Srivastava, City of Hope Scientist and 2017 participant.

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