Participants learn to:
– Establish and maintain a cell culture laboratory
– Select, operate, and maintain cell culture equipment
– Comply with biosafety regulations
– Reduce costs through optimized purchasing and laboratory organization
– Sterilize supplies
– Work aseptically
– Isolate cells by explant and enzymatic derivation procedures
– Prepare, aliquot and store cell culture reagents
– Passage, feed and assess adherent and non-adherent cells
– Count cells using a hemocytometer
– Plate cells in defined numbers for experimental procedures
– Cryopreserve cells
– Characterize cells via morphological and immunological markers
– Perform mycoplasma testing
– Electroporate cells with plasmid DNA
– Prevent and eliminate contaminations
– Troubleshoot common cell culture problems