Grade – 9th to 12th
|Format/ Schedule||After School / Winter and Fall|
|2020 Dates||Spring: Mar 4th, Mar 11th, Mar 18th, Mar 25th. Fall: Sept 16th, Sept 23rd, Sept 30th, Oct 7th.|
|Duration||4 x 2.5 Hour Sessions|
|Times||5.00pm-7.30pm, every Wednesday for 4 weeks. Weekend classes may be available (inquire for details)|
|Eligibility||Methods in Stem Cell Engineering is an introductory hands-on program, open to both residential and international students. It is suited to science enthusiasts with a basic understanding of cell and molecular biology. No prior laboratory experience is required to take this course. Applicants must have passed high-school Math, English and Biology or equivalent courses with a grade C or above. Students are required to arrange their own transportation to and from the program.|
|Admission||Eligible students are accepted on a first-come-first-served rolling basis up to 1 week before the course start date. Additional questions? Click here to learn more about our general admissions policy or call us at 310-483-3651.|
Methods in Stem Cell Engineering is a four-session afterschool program exploring the science of gene modulation in stem cells. Participating students learn basic hands-on lab skills, using RNA interference (RNAi) to silence the expression of an oncogene in human cancer stem cells.
RNAi is a sophisticated technique that scientists use to inhibit the expression of specific genes in order to study their function. During Methods in Stem Cell Engineering, students learn bioinformatical approaches to designing an RNAi experiment and measure the effect of inhibiting the gene oct-4 in stem cells derived from testicular cancers. They also explore the real-world applications of RNAi technology, hearing from experts in the field of stem cell engineering.
Each session of the course focuses on the following topics in stem cell engineering and gene silencing:
Session 1: Intro to cells, stem cells and RNAi gene silencing
– College-level lectures
*Intro to stem cell systems provides practical training in: BSL-2 lab safety procedures, aseptic technique, electroporation, RNAi mediated gene knockdown, antibody labeling and microscopy (phase contrast and fluorescent).